Oxygen requirement for prostaglandin biosynthesis

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Prostaglandins and Medicine 1: 117-120, 1978. OXYGEN REQUIREMENT FOR PROSTAGLANDIN BIOSYNTHESIS

The formation of prostaglandins by vesicular gland cyclooxygenase can be regulated by 02 concentrations below 30 11% (20 mm Hg) with an apparent Km value of about 5 PM. This result suggests that most mammalian tissues might be expected normally to have sufficient 02 for some synthesis, and that only occasionally, would the level of oxygen available within some tissues become marginally inadequa...

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Prostaglandin E synthase, a terminal enzyme for prostaglandin E2 biosynthesis.

Biosynthesis of prostanoids is regulated by three sequential enzymatic steps, namely phospholipase A2 enzymes, cyclooxygenase (COX) enzymes, and various lineagespecific terminal prostanoid synthases. Prostaglandin E synthase (PGES), which isomerizes COX-derived PGH2 specifically to PGE2, occurs in multiple forms with distinct enzymatic properties, expressions, localizations and functions. Two o...

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Microsomal Prostaglandin Biosynthesis

We have investigated prostaglandin biosynthesis by human renal medullary and cortical microsomes with arachidonic acid as substrate. Prostaglandins were measured by two independent methods, namely radiometric thin layer chromatography and radioimmunoassay. The relative abundance of various prostaglandins synthesized by microsomes was similar in cortex and medulla. In the absence of added cofact...

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Evaluation of oxygen requirement in patients during fiberoptic bronchoscopy

Background: Sometimes oxygen is given to patients during fiberoptic bronchoscopy to prevent arterial hypoxemia, but oxygen usually is given to patients with SPO2 less than 90%. The purpose of this study was to evaluate the patient's oxygen requirement according to SPO2 and its relationship with Pulmonary Function Test (PFT). Methods: From January 2006 to April 2007, 146 patients who needed fibe...

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Ionophores stimulate prostaglandin and thromboxane biosynthesis.

The role of calcium in triggering prostaglandin and thromboxane synthesis was studied in several systems with ionophores of different ion specificities. Divalent cationophore A23187 stimulates prostaglandin and thromboxane production by washed human platelets in a concentration-dependent manner (0.3-9 muM). A23187 also induces an antimycin A-insensitive burst in oxygen utilization which is part...

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ژورنال

عنوان ژورنال: Prostaglandins and Medicine

سال: 1978

ISSN: 0161-4630

DOI: 10.1016/0161-4630(78)90037-x